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2019;32(3):e4058

For evaluating snRNA levels in ascites and conditioned media samples, RT-qPCR was conducted in triplicate with BioMaster RT-PCR SYBR Blue (Biolabmix, RM04-200) on the LightCycler 96 System (Roche, Switzerland)

Please keep it in a cool, dry place, away from direct sunlight.

143B cells were plated in 12-well plates at a density of 3 10 5 cells/well and subsequently cultured for 12 h

One of the most interesting features of one carbon metabolism is that reaction velocities are often affected not just by substrate and product concentrations and enzyme activities but also by the concentrations of substrates in distant parts of the reaction network that act as allosteric activators or inhibitors of the enzyme